(3)取得的主要研究成果(获奖成果、论文、专利、著作等)
1)主要研究成果
“甘蓝型油菜自交不亲和两系杂种选育方法及其关键技术”在年度湖北科技创新“十佳”评选中,被评为“湖北十大科技转化成果”。
实验室发表论文27篇,其中SCI收录17篇。通过审定油菜新品种2个,其中圣光87通过国家农作物品种审定委员会审定,圣光901通过甘肃省农作物品种审定委员会审定。授权专利9个。
2)发表论文27篇,其中SCI收录论文17篇如下:
1、 Zhang XL, Ge XH,Li ZY. Genomic Change, Retrotransposon Mobilization and Extensive Cytosine Methylation Alteration in Brassica napus Introgressions from Two Intertribal Hybridizations . PLOS ONE,2013,8(2): e56346, DOI: 10.1371/journal. pone. 00563
2、 Lu W, Hong DF, Yang GS. A triallelic genetic male sterility locus in Brassica napus: an integrative strategy for its physical mapping and possible local chromosome evolution around it . ANNALS OF BOTANY,2013, 111 (2) : 305-315 DOI: 10.1093/aob/mcs260
3、 Liu YJ , Liu KD.Running MP. CCS52A2/FZR1, a cell cycle regulator, is an essential factor for shoot apical meristem maintenance in Arabidopsis thaliana .BMC PLANT BIOLOGY,2013,(12):135 DOI: 10.1186/1471-2229-12-135
4、 Zhang CY, Cahoon EB. Genetic and biochemical basis for alternative routes of tocotrienol biosynthesis for enhanced vitamin E antioxidant production .PLANT JOURNAL, 2013, 73 (4): 628-639 DOI: 10.1111/tpj.12067(IF=6.582)
5、 Li HT , Liu KD . Development of a core set of single-locus SSR markers for allotetraploid rapeseed. THEORETICAL AND APPLIED GENETICS, 2013, 126(4): 937-947 DOI: 10.1007/ s00122-012-2027-z
6、 Gao CB, Ma CZ. The genetic characterization of self- incompatibility in a Brassica napus line with promising breeding potential. MOLECULAR BREEDING, 2013,31(2): 485-493 DOI: 10.1007/s11032-012-9805-9
7、 Hou JN, Meng JL. A Tourist-like MITE insertion in the upstream region of the BnFLC.A10 gene is associated with vernalization requirement in rapeseed . BMC PLANT BIOLOGY,2013,(12): 238 DOI: 10.1186/1471-2229-12-238
8、 Cai GQ . Zhou YM .Identification of candidate genes of QTLs for seed weight in Brassica napus through comparative mapping among Arabidopsis and Brassica species.BMC GENETICS,2013,(13): 105 DOI: 10.1186/1471-2156-13-105
9、 Cheng, Y, Zhou YM . Downregulation of multiple CDK inhibitor ICK/KRP genes upregulates the E2F pathway and increases cell proliferation, and organ and seed sizes in Arabidopsis. PLANTJOURNAL75, 2013, (4):642-655 DOI:10.1111/tpj.12228
10、 Zhang, CY, Cahoon, EB. A thraustochytrid diacylglycerol acyltransferase 2 with broad substratespecificity strongly increases oleic acid content in engineered Arabidopsis thaliana seeds,JOURNAL OF EXPERIMENT AL BOTANY,2013, 64(11): 3189- 3200 DOI:1093/jxb/ert156
11、 Jian Wu, Guangqin Cai, Jiangying Tu, Lixia Li, Sheng Liu, Xinping Luo, Lipeng Zhou, Chuchuan Fan, Yongming Zhou ,Identification of QTLs for Resistance to Sclerotinia Stem Rot and BnaC.IGMT5.a as a Candidate Gene of the MajorResistant QTL SRC6 in Brassica napus. PLOS ONE 8(7): e67740. DOI:10.1371/ journal.pone.0067740
12、 Ge XH,Li ZY. Nucleolar dominance and different genome behaviors in hybrids and allopolyploids. Plant cell reports . 32(11):1661-1673 DOI: 10.1007/s00299-013-1475-5
13、 Cui, Cheng,Ge, Xianhong,Li, Zaiyun. Cytoplasmic and Genomic Effects on Non-Meiosis-Driven Genetic Changes in Brassica Hybrids and Allotetraploids from Pairwise Crosses of Three Cultivated Diploids .PLOS ONE ,2013, 8(5): e65078 DOI: 10.1371/journal.pone.0065078
14、 Zhang CY ,Lim YP . Transcriptionally and Phylogenetically Analyzing the P Protein Gene of Glycine Decarboxylase for Understanding the Evolution of C-3-C-4 Species in Brassicaceae , HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY2013, 54 (1): 89-89 DOI: 10.1007/s13580-013-0215-7
15、 Chen, X ,Liu, KD . Detection and genotyping of restriction fragment associated polymorphisms in polyploid crops with a pseudo-reference sequence: a case study in allotetraploid Brassica napus.BMCGENOMICS,2013,14DOI: 10.1186/1471-2164-14-346
16、 Zhao, YZ ,Liu, KD .Characterization and genetic mapping of a novel recessive genic male sterile gene in sesame (Sesamum indicum L.). MOLECULAR BREEDING ,2013, 32(4): 901-908 ,DOI: 10.1007/s11032-013-9919-8
17、 Li, FP ,Ma, CZ . Molecular cloning and mRNA expression profile of Sucrose Transporter Gene BnSUT1C from Brassica napus L. INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY ,2013, 51 (12): 1130-1136
3)获批专利
序号专利名称完成人专利号授权公告日
1. 过表达甘蓝型油菜BnLAS基因提高植物抗旱性周永明、杨明贵、杨庆勇ZL 2010 1 0623505.52013-05-08
2. 一种甘蓝型油菜低亚麻酸分子标记及其制备方法与应用周永明、傅廷栋 杨庆勇Zl 2011 1 0389151.72013-05-08
3. 甘蓝型油菜粒重主效QTLs的分子标记及其应用周永明、傅廷栋、范楚川、蔡光勤ZL 2010 1 0120725.62013-03-27
4. 甘蓝型油菜粒重粒子重相关基因的特异分子标记及应用周永明、傅廷栋、范楚川、蔡光勤ZL 2010 1 0169042.X2013-03-27
5. 一种甘蓝型油菜粒重性状的分子标记及制备方法与应用周永明;傅廷栋;范楚川;蔡光勤ZL 2011 1 0374200.X2013-11-6
6. 小孢子培养方法选育油菜温敏型波里马细胞质雄性不育两用系的方法杨光圣等ZL 2011 1 0099605.72013-5-8
7. 人工辅助授粉自交方式选育油菜温敏型波里马细胞质雄性不育两用系的方法杨光圣等ZL 2011 1 0099759.62013-4-10
8. 一种甘蓝型油菜萝卜甘蓝细胞质雄性不育系的选育方法吴江生;石淑稳;刘克德;王小峰;刘超;ZL 2010 1 0557897.X2013-11-6
9. 一种甘蓝型油菜芥菜细胞质雄性不育系的选育方法吴江生;石淑稳;刘克德;刘超;王小峰20101057077922013-4-10
4)审定品种
序号成果名称审定或鉴定单位时间完成人
1圣光901(甘审油2013004)甘肃省农作物品种审定委员会2013-3-26杨光圣
2圣光87国家农作物品种审定委员会2013杨光圣
3、对学科发展的贡献
1)新开发的一批分子标记将用于油菜重要性状的遗传改良,可加快育种进程。
2)克隆的油菜核不育基因及其功能的阐述,将为进一步明确雄性不育的遗传和分子机理提供新信息。
3)有关亚基因组杂种优势机理研究,一方面可提供有价值的育种资源,另一方面可为阐明杂种优势机理提供新知识。
4)有关抗逆性状的遗传及基因定位,可为进一步深入了解油菜及油料作物的生态适应性奠定理论基础。
5)定位和与种子发育及品质性状有关基因可为高产优质育种提供基因资源、育种工具。
6)基因组分析结果对全面深入了解芸薹属物种的起源和进化,挖掘新型育种资源奠定基础。